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goscripttm 5x rt reaction buffer  (Promega)

 
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    Promega goscripttm 5x rt reaction buffer
    Goscripttm 5x Rt Reaction Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/5x+goscripttm+buffer/goscript+5x+reaction+buffer/pmc11680345-65-10-15
    Average 90 stars, based on 1 article reviews
    goscripttm 5x rt reaction buffer - by Bioz Stars, 2026-09
    90/100 stars

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    Reverse Transcription:

    Article Title: Nutritional status affects Igf1 regulation of skeletal muscle myogenesis, myostatin, and myofibrillar protein degradation pathways in gopher rockfish (Sebastes carnatus).
    Article Snippet: Total RNA was DNase I treated (TURBO DNA-free Kit, Ambion) and quantified by spectrophotometry (P300 NanoPhotometer, Implen; 260:280 ratios ≥1.96). .. Total RNA was then reverse transcribed using 5.6 μl of 5x GoScriptTM Buffer, 4.2 μl of MgCl2 (25 mM), 1.4 μl dNTPs (10 mM, Promega Corp., Madison, WI, USA), 1.4 μl random hexamer primers (500 μg/ml; Promega Corp.), 0.11 μl recombinant RNasin ribonuclease inhibitor (40 Units/μl; Promega Corp.), 0.25 μl nuclease-free H2O, 1.05 μl GoScriptTM reverse transcriptase (Promega Corp.), and 14 μl of RNA template (30.0 ng/μl for liver RNA, 32.5 ng/μl for muscle RNA) under a thermal profile of 25 ◦C for 5 min and 42 ◦C for 1 h, followed by 70 ◦C for 15 min. Real-time quantitative PCR was used to measure relative mRNA levels of genes in liver and skeletal muscle. .. All quantitative PCR reactions were run as 10 μl volumes containing 5 μl of iTaqTM Universal SYBR Green Supermix (BioRad Laboratories, Inc.), 1.5 μl of nucleasefree H2O, 1.5 μl of cDNA, and 1 μl each of forward primer (10 μM) and reverse primer (10 μM).

    Random Hexamer:

    Article Title: Nutritional status affects Igf1 regulation of skeletal muscle myogenesis, myostatin, and myofibrillar protein degradation pathways in gopher rockfish (Sebastes carnatus).
    Article Snippet: Total RNA was DNase I treated (TURBO DNA-free Kit, Ambion) and quantified by spectrophotometry (P300 NanoPhotometer, Implen; 260:280 ratios ≥1.96). .. Total RNA was then reverse transcribed using 5.6 μl of 5x GoScriptTM Buffer, 4.2 μl of MgCl2 (25 mM), 1.4 μl dNTPs (10 mM, Promega Corp., Madison, WI, USA), 1.4 μl random hexamer primers (500 μg/ml; Promega Corp.), 0.11 μl recombinant RNasin ribonuclease inhibitor (40 Units/μl; Promega Corp.), 0.25 μl nuclease-free H2O, 1.05 μl GoScriptTM reverse transcriptase (Promega Corp.), and 14 μl of RNA template (30.0 ng/μl for liver RNA, 32.5 ng/μl for muscle RNA) under a thermal profile of 25 ◦C for 5 min and 42 ◦C for 1 h, followed by 70 ◦C for 15 min. Real-time quantitative PCR was used to measure relative mRNA levels of genes in liver and skeletal muscle. .. All quantitative PCR reactions were run as 10 μl volumes containing 5 μl of iTaqTM Universal SYBR Green Supermix (BioRad Laboratories, Inc.), 1.5 μl of nucleasefree H2O, 1.5 μl of cDNA, and 1 μl each of forward primer (10 μM) and reverse primer (10 μM).

    Recombinant:

    Article Title: Nutritional status affects Igf1 regulation of skeletal muscle myogenesis, myostatin, and myofibrillar protein degradation pathways in gopher rockfish (Sebastes carnatus).
    Article Snippet: Total RNA was DNase I treated (TURBO DNA-free Kit, Ambion) and quantified by spectrophotometry (P300 NanoPhotometer, Implen; 260:280 ratios ≥1.96). .. Total RNA was then reverse transcribed using 5.6 μl of 5x GoScriptTM Buffer, 4.2 μl of MgCl2 (25 mM), 1.4 μl dNTPs (10 mM, Promega Corp., Madison, WI, USA), 1.4 μl random hexamer primers (500 μg/ml; Promega Corp.), 0.11 μl recombinant RNasin ribonuclease inhibitor (40 Units/μl; Promega Corp.), 0.25 μl nuclease-free H2O, 1.05 μl GoScriptTM reverse transcriptase (Promega Corp.), and 14 μl of RNA template (30.0 ng/μl for liver RNA, 32.5 ng/μl for muscle RNA) under a thermal profile of 25 ◦C for 5 min and 42 ◦C for 1 h, followed by 70 ◦C for 15 min. Real-time quantitative PCR was used to measure relative mRNA levels of genes in liver and skeletal muscle. .. All quantitative PCR reactions were run as 10 μl volumes containing 5 μl of iTaqTM Universal SYBR Green Supermix (BioRad Laboratories, Inc.), 1.5 μl of nucleasefree H2O, 1.5 μl of cDNA, and 1 μl each of forward primer (10 μM) and reverse primer (10 μM).

    Real-time Polymerase Chain Reaction:

    Article Title: Nutritional status affects Igf1 regulation of skeletal muscle myogenesis, myostatin, and myofibrillar protein degradation pathways in gopher rockfish (Sebastes carnatus).
    Article Snippet: Total RNA was DNase I treated (TURBO DNA-free Kit, Ambion) and quantified by spectrophotometry (P300 NanoPhotometer, Implen; 260:280 ratios ≥1.96). .. Total RNA was then reverse transcribed using 5.6 μl of 5x GoScriptTM Buffer, 4.2 μl of MgCl2 (25 mM), 1.4 μl dNTPs (10 mM, Promega Corp., Madison, WI, USA), 1.4 μl random hexamer primers (500 μg/ml; Promega Corp.), 0.11 μl recombinant RNasin ribonuclease inhibitor (40 Units/μl; Promega Corp.), 0.25 μl nuclease-free H2O, 1.05 μl GoScriptTM reverse transcriptase (Promega Corp.), and 14 μl of RNA template (30.0 ng/μl for liver RNA, 32.5 ng/μl for muscle RNA) under a thermal profile of 25 ◦C for 5 min and 42 ◦C for 1 h, followed by 70 ◦C for 15 min. Real-time quantitative PCR was used to measure relative mRNA levels of genes in liver and skeletal muscle. .. All quantitative PCR reactions were run as 10 μl volumes containing 5 μl of iTaqTM Universal SYBR Green Supermix (BioRad Laboratories, Inc.), 1.5 μl of nucleasefree H2O, 1.5 μl of cDNA, and 1 μl each of forward primer (10 μM) and reverse primer (10 μM).



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